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BD FACSDiva Installation

Last Updated: January 31, 2018

Backup old files​

Backup BD Database using Data Utility

Backup D:\BD and C:\ProgramData\BD (contains configurations)

Export configurations as csv just in case. Launch CST and export configurations.

Installation​

This assumes you are using the provided BD Computer.

  1. Install 32-bit Windows 7 SP1

    • make sure you have SP1

  2. Install drivers (BD-provided computer)

    • Firepro v3900 GPU
    • Intel NICs
    • Will install license key driver automatically

  3. Install DIVA

    • Make sure Firewall is off (firewall config will happen after the program installs

    • Turn on Cytometer, wait about 2 minutes for it to boot

      • Can watch progress through PuTTY on COM1

    • Select LSRFortessa and High Throughput sampler

    • Will load firmware

      • If that fails, check firewall, connection through PuTTY, and if its joined to the domain (I installed DIVA before joining it)

    • Once it is done, restart.

    • Restore the database through the Data Utility

      • Other stuff needs to happen other than just copying over the files.

  4. Join to Domain

  5. Reboot

  6. Verify these tasks:

    • Log into DIVA
    • Connect to cytometer
    • Access CST
      • Verify that the base configuration is correct

Troubleshooting​

Permission issues on Login or on CST​

  • Make sure that C:\ProgramData\BD\Shared and C:\ProgramData\BD\FASCDiva have Users & System granted full control

No connection to cytometer​

  • Ping 192.168.1.1
  • Verify PuTTY
  • Verify that Java can traverse firewall (Cytometer network is usually on public profile)

Other Notes​

  • It uses FTP to transfer firmware and configuration to the cytometer

64-bit​

Can be installed on a 64-bit machine but 32-bit java requires a contiguous chunk of RAM. You'll need to have a quite a bit of free memory available to do this or you might run into issues starting it.